Transgenic / Gene Targeting Facility

In vitro fertilization to rederive mouse lines from frozen or fresh sperm.

Services provided by the facility:

Hormone induced eggs from either CBA x C57Bl6 F1 mice or C57Bl6 inbred mice.

Pseudopregnant females to implant embryos. 

Housing for mice until weaning.

Responsibilities of the investigator:

· Ampoule of frozen sperm or a male mouse.

· Submit a “service request form”.

Rederivation of Mouse Line via IVF fee includes:

$835 for rederivation of genetically altered mice from frozen or fresh sperm.  $1200 will be charged if using C57Bl6 eggs.

Any animals left with the facility longer than 3 weeks after tail biopsies have been returned for genotyping will be charged to the investigator at $2.00/ day/ cage of animals.

IVF

IVF