Transgenic / Gene Targeting Facility

ES Cell Targeting

Production of gene targeted embryonic stem cells.  We can target your DNA into either 129 derived R1 ES cells or G4 ES cells which are a 129/C57Bl6 hybrid.

Price: $5000

 

Guaranteed 100 or more clones.

Services provided by the facility:

The gene-targeting facility will electroporate DNA provided by the investigator into embryonic stem cells (ES cells) and perform positive-negative selection.  DNA from surviving clones will be returned to the investigator to assay for targeted clones. 

Responsibilities of the investigator:

· Discuss experimental design with facility director before initiation of the project.

· Provide a map of the construct including size, regions of homology, selection strategy, enzyme sites, primer binding sites.

· Purify the DNA targeting vector according to protocols provided by the facility. We’ll need 50ug of purified, linearized DNA at ~1ug/ul.

· Provide gel documentation of the purity and concentration of the DNA target.

· Develop a reliable genotyping assay before submission of DNA to the facility.

· Submit a “service request form”.

· Frozen ES cells will be stored for two months while the DNA is being analyzed.

 

Gene Targeted ES Cell Production Fee includes:

$5000 will be charged upon delivery of DNA from at least 100 clones.

A yearly storage fee of $50 will be charged for 1-50 vials of ES cells, $100 for 51-100 vials, and $150 for 101-150 vials.

Production of gene-targeted ES cells.