Transgenic / Gene Targeting Facility

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ES Cell Targeting

Production of gene targeted embryonic stem cells.  We can target your DNA into either 129 derived R1 ES cells or G4 ES cells which are a 129/C57Bl6 hybrid.

Price: $5000

 

Guaranteed 100 or more clones.

Services provided by the facility:

The gene-targeting facility will electroporate DNA provided by the investigator into embryonic stem cells (ES cells) and perform positive-negative selection.  DNA from surviving clones will be returned to the investigator to assay for targeted clones. 

Responsibilities of the investigator:

· Discuss experimental design with facility director before initiation of the project.

· Provide a map of the construct including size, regions of homology, selection strategy, enzyme sites, primer binding sites.

· Purify the DNA targeting vector according to protocols provided by the facility. We’ll need 50ug of purified, linearized DNA at ~1ug/ul.

· Provide gel documentation of the purity and concentration of the DNA target.

· Develop a reliable genotyping assay before submission of DNA to the facility.

· Submit a “service request form”.

· Frozen ES cells will be stored for two months while the DNA is being analyzed.

 

Gene Targeted ES Cell Production Fee includes:

$5000 will be charged upon delivery of DNA from at least 100 clones.

A yearly storage fee of $50 will be charged for 1-50 vials of ES cells, $100 for 51-100 vials, and $150 for 101-150 vials.

Production of gene-targeted ES cells.

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