Transgenic / Gene Targeting Facility

Cryopreservation of mouse embryos using LN2.

Services provided by the facility:

Freezing and storage of embryos.

Thawing of single straw for viability test.

Responsibilities of the investigator:

· Provide 10 superovulated mated female mice of breeding age.

· Provide us with the following information before day of freezing:

                         Genetic name of the mouse

                         Whether it is a transgenic or targeted line

                         Mouse number (your number)

                         Birthdate

                         Genotype (homozygous or heterozygous)

                         Mouse background strain

 

· Submit a “service request form”.

Embryo Freezing Fee includes:

Dissection of females and collection of embryos.

Freezing of 100-150 embryos in straws using our rate controlled freezer.

Thawing of one straw two weeks after freezing for viability check.

A yearly storage fee of $50 will be charged for 1-50 straws of embryos and $100 for 51-100 straws.

Embryo Freezing

Embryo Freezing