Blastocyst Injection

Production of chimeric mice using ES cell injection into blastocysts.

Price: $5000

 

Guaranteed 2 chimeric males 80% or better

Services provided by the facility:

Selected targeted clones will be microinjected into C57Bl/6 blastocysts, after which they will be implanted into pseudopregnant females.  Any resulting male pups which are >40% chimeric will be bred for possible germline transmission by the facility. 

Responsibilities of the investigator:

· Discuss experimental design with facility director before initiation of the                   project.

· Provide targeted ES cells or clone number of positives.

· Develop a reliable genotyping assay before submission of ES cells to the                facility.

· Obtain IACUC approval for your project before submission of ES cells.

· Obtain space with Animal Resources Center for housing targeted animals.

· Assign personnel within the investigator’s lab to monitor targeted animals.

· Submit a “service request form”.

 

Chimeric Mice Production Fee includes:

      $5000 fee will be charged upon production of 2 or more >80% male chimeras.

      Any animals left with the facility longer than 3 weeks after tail biopsies have been       returned for genotyping will be charged to the investigator at $2.00/ day/ cage of        animals.

Gene-targeted Mouse Production

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