HSC Core Research Facilities
Transgenic/Gene-targeting Mouse Facility
Main
The Facility is available to make transgenic mice using pronuclear injection of embryos as well as gene-targeting of ES cells and blastocyst injection. The Transgenic and Gene-targeting Core offers related procedures including sperm freezing, embryo freezing, rederivation of mouse lines, IVF, karyotyping, and has added new services including ICSI (intracytoplasmic sperm injection) and development of ES cell lines from your mouse line. We have successfully provided genetically altered mice to University of Utah researchers via frozen sperm and frozen embryos shipped from all over the world.
The Core is available for technical advice regarding injection procedures, cell culture techniques, vector design and construction; and we are open to new ideas and available to try new methods that you are interested in. The Core maintains the necessary mouse colonies for basic procedures, including a colony of flp mice and EYFP mice. We maintain 129 ES cells as well as 129/C56B16 hybrid ES cells for gene-targeting.
