HSC Core Research Facilities
Mass Spectrometry and Proteomics Core*
(link to Mass Spectrometry and Proteomics Core Home page)Main
This core provides mass spectrometry services and consultation for the University of Utah and Huntsman Cancer Institute (HCI) research communities, and operates as a fee-for-service resource. Off-campus samples from academic institutions or private companies are accepted, as well. While the primary emphasis of the core is geared toward supporting proteomics research, the facility continues to provide basic MS support for a broad range of research and sample types, such as polymers, natural products, small synthetic molecules, and large intact proteins and nucleic acids. Our mission is to provide the highest quality mass spectrometry analyses for protein and other biomolecule investigations.
The facility is equipped with several high-performace mass spectrometers, including a new state-of-the-art FTMS(LTQ-FT, ThermoElectron), with both ESI and Maldi ionization capabilities. especially suitable for proteomics applications, the FTMS instrument provides high sensitivity (sub-femtomole), high resolution (>500,000), and high mass accuracy (<2ppm), affording greater sequence information and protein ID. The FTMS is well equipped for mapping protein modifications, with CID, IRMPD, and ECD fragmentation techniques. LC/MS/MS instruments are equipped with nano-LC, for ultimate sensitivity and chromatographic performance.
A range of proteomics services include: protein ID from in-gel or solution, using LC/MS/MS, FTMS, or Maldi/ToF; protein ID from complex cell isolates; post-translational modifications (PTMs such as phosphorylation, GIcNAc, acetylation); and in-house Mascot database searching (public or custom DBs); and ESI/MS for intact protein MW and identification of truncation sites.
*This is a designated Huntsman Cancer Institute CCSG - Shared Resource
