HSC Core Research Facilities
Genomics Facility
Main
The Genomics Core Facility offers a variety of genetic analysis services to both on-campus and off-campus researchers, including full service genotyping, from PCR setup through analysis, and assistance to researchers performing genotyping projects. Their facility has commercial and custom sets of fluorescently labeled microsatellite markers that can be used for whole genome linkage studies and fine mapping projects.
Particular sets of markers can be used for loss of heterozygosity and microsatellite instability studies. An Applied Biosystems 3130xl instrument is used to run genotyping samples and Applied Biosystems GeneMapper software is used for data analysis. The 3130xl instrument is also available for researchers with fluorescently labeled PCR products that are ready to run. SNP genotyping is available using Applied Biosystems Taqman genotyping assays along with higher throughput GoldenGate assays from Illumina on the BeadXpress reader. For very high throughput or GWAS applications, the core has the Illumina iScan with the capability of running any iScan chip Illumina offers, this includes chips available for mouse, rat, human, and other subjects.
Two Applied Biosystems 7900HT instruments are available for researchers interested in performing real time PCR experiments for gene expression studies. A Biomek FX and a Velocity 11 VPrep robot are available to researchers that require automated pipetting for their experiments. In addition to standard DNA sequencing, the Genomics facility offers a mutation detection service to identify single nucleotide polymorphisms (SNPs), insertions, and deletions.
Researchers can select genes or regions of interest and the facility designs and optimizes the PCR primers, performs the initial PCR, runs the sequencing reactions, and analyzes the data using SoftGenetics Mutation Surveyor software.
