Cell Imaging...Microinjection
Contact info: Chris Rodesch crodesch@cores.utah.edu Phone: 587-7964 Fax: 585-6364	Back to Cell Imaging Home

two automated Eppendorf Microinjection systems for cytoplasmic and nuclear injection of dissolved substances manipulation of microcapillary into target cell and dissolved substance injection are combined into a single, automated operation cell viability and injection reproducability are optimized used with a wide array of injectates, including oligonucleotides, short peptides, full length DNA and RNA, proteins, antibodies, and various dyes and fluorescent injection markers

Examples: Cytoplasmic Microinjection:

Kinetics and morphology of apoptosis induced by mannitol in skeletal muscle cells was analyzed. C2C12 mouse muscle cells were microinjected with a rhodamine red dye. Myotubes are shown before (A) and after (B) hyperosmolar shock with 300 mM mannitol. Mannitol causes loss of myotubular morphology consistent with apoptosis. Images and protocol courtesy Brandi Baker and Dr. J. Rob Singleton, Department of Neurology.

Nuclear Microinjection:

A DNA construct containing the sequence for green fluorescent protein (GFP) was microinjected into HeLa cells. Following microinjection, cells were incubated for 18 hours, then examined for the presence of GFP. Cells remained viable after injection, and were able to successfully transcribe the injected sequence and produce the fluorescent protein. Cells and GFP construct courtesy Mr. Michael Vaughn and Dr. Jerry Kaplan, Department of Pathology.

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