LCQ-Deca (ThermoElectron) 
The LCQ-Deca is an ion-trap mass spectrometer with electrospray (ESI) ionization and MS/MS or MSnth capabilities. This instrument is primarily dedicated for protein ID, analysis of peptides, and other proteomics work, but is also utilized for LC/MS/MS of small-molecule samples that require LC introduction. It is equipped with a nanoLC system (Eksigent) and is routinely used for protein ID from in-gel digests and proteins from solution.
For applications of the instrument, see available services.
LCQ-Deca Performance and Capabilities
- Nano-LC/MS/MS
- < 0.5 Da mass error (full scan);
<0.05 Da mass error (zoom scan) - Femtomole-range sensitivity (e.g. 2 femtomoles BSA digest injected on column)
- MS/MS with CID fragmentation (for peptide sequencing or structure elucidation)
How the LCQ-Deca is Equipped
- Nanospray or microspray ESI
- NanoLC system (Eksigent Technologies) –
- splitless gradients as low as 20 nl/min; 75um ID C18 column;
- protein digests typically analyzed at a flow rate of 400nl/min
- Collision-induced dissociation (CID) for MS/MS
The facility is also equipped with several other instruments:
