Other Equipment and Software
- Mascot software and license:
- Mascot protein database searching software and peptide denovo sequencing
- 2-processor Mascot license for in-house computer searches
- PEAKS (BSI) software and license:
- Peptide denovo sequencing and protein database searching software
- PEAKS license for in-house computer searches
- Sequest software and license:
- Sequest protein database searching software for in-house searches
- ThermoElectron software and licenses on the LTQ-FT:
- Excalibur 1.4 SRI, Bioworks 3.2, ProMass 2.5, Salsa, Xtract , Sequest.
- Waters/Micromass software:
- Masslynx, Biolynx, MaxEnt software for analyzing protein data on the Quattro-II
- MaxEntTM deconvolution software is for processing multiply-charged ESI data into
neutral molecular mass spectra
- Workstation computers for users to review data if desired
- HPLC systems:
- Two Eksigent 1D nanoLC systems (interfaced on LCQ and LTQ-FT instruments)
- splitless nanoLC, gradients as low as 20 nl/min
- 75um ID, 3um C18 packed-tip columns
- typical flow rate 400 nl/min for analysis of protein digests
- peptide peak widths 3 to 4 seconds wide at half-height
- Two HP1100 systems (not equipped with on-line UV detectors)
- Shimadzu LC-10AD system
- Can be interfaced with Quattro-II mass spectrometer for microspray applications (i.e. 3 to 10 ul/min flow rates)
- Not equipped with an on-line UV detector
- Mascot protein database searching software and peptide denovo sequencing
- 2-processor Mascot license for in-house computer searches
- Peptide denovo sequencing and protein database searching software
- PEAKS license for in-house computer searches
- Sequest protein database searching software for in-house searches
- Excalibur 1.4 SRI, Bioworks 3.2, ProMass 2.5, Salsa, Xtract , Sequest.
- Masslynx, Biolynx, MaxEnt software for analyzing protein data on the Quattro-II
- MaxEntTM deconvolution software is for processing multiply-charged ESI data into neutral molecular mass spectra
- Two Eksigent 1D nanoLC systems (interfaced on LCQ and LTQ-FT instruments)
- splitless nanoLC, gradients as low as 20 nl/min
- 75um ID, 3um C18 packed-tip columns
- typical flow rate 400 nl/min for analysis of protein digests
- peptide peak widths 3 to 4 seconds wide at half-height
- Two HP1100 systems (not equipped with on-line UV detectors)
- Shimadzu LC-10AD system
- Can be interfaced with Quattro-II mass spectrometer for microspray applications (i.e. 3 to 10 ul/min flow rates)
- Not equipped with an on-line UV detector
The facility is also equipped with several other instruments:
