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FTMS Services

FTMS is available with the following sample-introduction methods:

  • nanoLC/MS and nanoLC/MS/MS
  • AP-Maldi (MW limited to < 4000 MW; mass accuracy above 2000MW is <10ppm)
  • Nanoflow ESI
  • Static nanospray ESI (pico-tip)
  • Microspray ESI


FTMS Service What is Performed at the Facility
Accurate mass measurement – external calibration
(Positive-ion) < 2ppm mass error
Positive-ion ESI measurement, infusion of the sample,
external standard calibration; mass accuracy better than 2 ppm
  1. Ziptip purification, if required
  2. ESI/MS, infusion of sample
  3. mass measurement with
    < 2ppm error
  4. generate list of possible compositions
Accurate mass measurement – internal calibration
(Positive-ion) < 1ppm mass error
Positive-ion ESI measurement, infusion of the sample,
internal standard calibration with at least one std;
mass accuracy better than 1 ppm
  1. Ziptip purification, if required
  2. ESI/MS, infusion of sample and internal standard
  3. mass measurement with
    < 1ppm error
  4. generate list of possible compositions
Accurate mass measurement –
(Negative-ion) < 2ppm mass error
Negative-ion ESI measurement, infusion of the sample,
external calibration; mass accuracy better than 2 ppm
 Inquire
Peptide Sequencing with MS/MS
and accurate mass denovo sequencing
CID, ECD, or IRMPD fragmentation methods available with FTMS analysis of the product ions

Sample introduced by infusion ESI or nanospray ESI (pico-tip);
Samples can also be ionized using AP-MALDI
Mascot or PEAKS sequence prediction search
 see Proteomics Services
“Top-Down” Proteomics
ECD fragmentation and sequencing of intact proteins
 Inquire
Identification of Sulfur-containing peptides
(i.e. containing cysteine or methionine )
FTMS with high resolution separation of heavy isotopes, (e.g. 13C and 34S isotopes; limited to peptides < 1000 MW)
 see Proteomics Services
Intact Protein MW Analysis
ESI/FTMS analysis and deconvolution of data using Xtract or ProMass software
  1. Ziptip purification, usually required
  2. ESI/FTMS analysis
  3. Xtract or ProMass deconvolution of data into a neutral molecular-mass spectrum
  4. Search protein sequence for truncation, cut sites, degradation products, or sequence modification with Biolynx software (if requested)
Special Requests for FTMS Analysis Inquire

Use the links below to learn about specific services:

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